Study on Impedimetric Measurement of Blood Cells Using ZnO-Nanowire Interdigitated Electrodes

• Main Authors: YI-CHUNG HUANG, 黃義中
• Other Authors: Yaw-Jen Chang
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/cpzzv4

碩士 === 中原大學 === 機械工程研究所 === 104 === Abstract In this thesis, a printed circuit boards (PCB) was treated as the chip substrate to allow FR4 copper foil to be patterned into copper interdigitated electrodes by photolithography process. Different types of photoresists were utilized to define the location of Zinc oxide (ZnO) nanowire selectively into the gap of copper interdigitated electrodes. Electrochemical impedance of erythrocytes was detected and analyzed via impedance meter and the recorded Nyquist plot. The results show that the electrochemical impedance spectra are distinguishable and more sensitive in comparison with those of interdigitated electrodes without ZnO nanowire. ZnO nanowire interdigitated electrodes can be exercised to detect the impedance of 3% red blood solution with a trace volume of 0.2ul. In different volume levels, the value can still be detected. The increase of probe volume provides larger impedance value which indicates the blood cells have larger impedance than the solvent. The impedance value of blood cell can be obtained from measuring with the nanowire interdigitated electrodes system. A simulated equivalent circuit of resistor(s) and capacitor(s) can be derived from the recorded Nyquist plot. Following with the measured value of solution resistance and the Electrical Double-Layer capacitance, the impedance of red blood cells can be calculated by deduction.