| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 104 === Artificial antibacterial agents (such as Methyl Peraben(M.P.) and Propyl Paraben(P.P.)) were important factor in causing skin allergies and skin irritation. Therefore, searching for alternatives and decreases of the artificial antibacterial agents have become a major issue in cosmetics industry. The interactions between antibiotic medicines and plant components have already been investigated by domestic and foreign scholars. Nonetheless, there isn’t relevant reports that relative to interactions between artificial agents in cosmetics and the extracts of Chinese herbal. This study evaluates the in-vitro interactions (synergism, addition effect, antagonism ) between methanol extracts of Pogostemon cablin, Almond acid and two kinds of common ingredients used in cosmetics, against Escherichia coli and Propionibacterium acne. The results of this study were expected that as a reference of type and that dose for adding antibacterial agents in cosmetics and Chinese herbals in order to reduce damage to human skin.
In this study, we extracted Pogostemon cablin with methanol, then practiced respectively the alone and one-on-one combined Antibacterial Susceptibility Testing (AST) of four kinds of reagents (Pogostemon cablin, almond acid, M.P. and P.P.) of vary concentrations (0.1 %, 1.0 %, 2.0 % and so on) with the Disk-diffusion method, and at last assessed the interactions between these four reagents with the Combination Index (CI).
The results of this study showed that Pogostemon cablin possessed good synergism or addition effects when it was combined respectively with Almond acid, M.P. and P.P.. This means that the formula amounts of M.P. and P.P. can be reduced and substituted by the increased using of Pogostemon cablin extracts and Almond acid in cosmetics, and the overall antibacterial ability of cosmetics could still be able to maintain. In addition to assess for anti-pimples of cosmetics in dose study that Almond acid which is commonly used could be replaced by adding more ingredients of Pogostemon cablin, and the best combination formula is 1.0 % Pogostemon cablin to 0.1 % Almond acid. As for the components of combined artificial antibacterial agents, M.P. and P.P., this study indicated that there is obvious antibacterial synergism while combining these two artificial antibacterial agents. Thus, even without the addition of Chinese herbal extracts; the original amount of M.P. and P.P. can be moderately reduced.
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