| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 104 === Sarcandra glabra also known as grass coral, guanyin tea or elderberry is an evergreen shrub plant containing clorantha lactone A and D, tannins, succinate, etc. Published literatures have shown that Sarcandra glabra with enhanced immune function and efficacy can get rid of rheumatism. Recent studies have also reported that Sarcandra glabra have the ability to promote healing and inhibit the growth and reproduction ability of tumor cells in mice. Researches have shown that the herb can increase catalase enzyme activity of cancer cells in mice and improve tumor-bearing capacity of the body’s oxygen consumption which has a direct inhibitory effect on cancer cells. This study was to use herbal plants sarcandra repair component as acne vulgaris, and further improve the effectiveness of herbs to detect the formation of acne factors.
This feasibility study presented five stages of experiments to explore the ability of Sarcandra glabra herbal exctract as a repair component in acne vulgaris. The drug’s inhibitory effect, cell viability, anti-inflammatory and anti-oxidative response capability (free radicals) in the treatment of acne vulgaris were assessed. The sample was prepared by shredding the rhizomal parts of the herbal plant using ethanol followed by brewing. The test drug was extracted using ultrasound-assisted Soxhlet extraction.Antibacterial efficacy experiments for Propionibacterium acne and Staphylococcus aureus Rosenbach were performed and RAW264.7 macrophage cell line was used for cell viability test (MTT assay). The inhibitory effect of the test drug for NO release was also studied using Nitric Oxide Assay followed by anti-free radical DPPH test. Where by the five stages of the experiment, the feasibility of herbal plant extracts applied sarcandra repair acne vulgaris.
Experimental results showed that sarcandra glabra extracts was able to effectively inhibit bacterial activities of propionibacterium acne and staphylococcus aureus. Comparison of the increasing dilution in the five test groups(0.5%,0.25%,0.125%,0.0625%,0.03125%) and control group in terms of cell viability, anti-inflammatory and antioxidative capacity showed significant improvements.MTT assay cell activation ability and DPPH radical scavenging activity are to enhance the efficiency of 3 times.Anti-inflammatory cell experiments induced by LPS showed inflammatory response, but after the addition of herbs, which can achieve above 90% against of the resistance to toxic substances and play a defense mechanism to protect the cells.
Chinese herbal skin care products research and development has been a high-profile especially for the treatment of acne vulgaris. Long-term treatment of acne with chemical drugs(eg:antibiotics) will produce drug-resistant bacteria and cause medical problems in the future and mild herbal ingredients resistant scruples low, therefore the results of this study can be used as a product development reference in the treatment of acne vulgaris.
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