The Study of Neuronal Differentiation of Rat Bone Marrow Mesenchymal Stem Cells on Poly-(o-methoxyaniline) / Poly(ε-caprolactone) Coaxial Electrospun Fibers

• Main Authors: Chi-Jung Tsai, 蔡季蓉
• Other Authors: Wen-tyng Li
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/p9g4jw

碩士 === 中原大學 === 生物醫學工程研究所 === 104 === Conductive polymers such as polyaniline and polypyrrole are known for their difficulty for fiber processing. With coaxial electrospinning, core-shell fibers may comprise of an electrospinnable core material and a non-electrospinnable shell material. Here, core–shell structured poly(ε-caprolactone) (PCL)-poly(o-methoxyaniline) (POMA) (PA/PC) nanofibers were successfully prepared by coaxial electrospinning technique. PA/PC fibers were doped with camphorsulfonic acid (CPSA) to form C-PA/PC nanofibers. Cell proliferation and neural differentiation of mesenchymal stem cells (MSCs) from rat bone marrow were studied on PA/PC and C-PA/PC nanofibers. Transmission Electron Microscopy (TEM) images showed that the diameters of PA/PC and C-PA/PC nanofibers were 194±37nm (core: 177±6 nm; shell: 14±3 nm) and 199±31 nm (core: 172±8 nm; shell: 16±2 nm), respectively. Fourier Transform Infrared (FTIR) spectroscopy and Thermogravity Analysis (TGA) showed that coaxial electrospun nanofibers had characteristic wave numbers and Decomposition temperature (Td) of POMA and PCL. Contact angle analysis revealed that CPSA doping enhanced surface hydrophilicity of PA/PC nanofibers. Cyclic voltammetry (CV) analysis demonstrated electroactivity of PA/PC and C-PA/PC nanofibers. Four point probe analysis found that C-PA/PC nanofibers had higher conductivity than PA/PC nanofibers. Scanning Electron Microscopy (SEM) images showed that both nanofibers were not biodegradable. The fibers had swollen appearance after long-term immersion in culture media. Mechanical analysis at macroscopic and microscopic level found that PA/PC nanofibers had better ductility than C-PA/PC nanofibers. MTS, fluorescence staining and SEM analyses exhibited improved attachment and proliferation of MSCs on both types of nanofibers. It is feasible to use core–shell nanofibers as a scaffold for neural differentiation of MSCs as demonstrated by immunostaining with neuronal cell markers. C-PA/PC nanofibers had higher expression on early neural differentiation marker-β III tubulin, whereas PA/PC nanofibers had higher expression on late neural differentiation marker-MAP-II.