| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 104 === Aging has always been a big issue for human being, and there is growing concern about the problem of cutaneous aging. Therefore, cosmetic products preventing cutaneous aging has increased significantly. This study focuses on photoaging, which is caused by UV radiation. It has been well known that photoaging includes several pathways, such as, oxidative stress, inflammation and apoptosis. In this study, puerarin, a natural extract from Pueraria lobata, is used to prevent photoaging. In view of the poor bioavailability of puerarin without a drug carrier, a liposomal dosage has been chosen to increase the biovailability of puerarin. Organic solvent is used to extract the puerarin, and solid phase extraction is used to purify the puerarin. The purity of isoflavone is found to be 98.5 % and the purity of puerarin is found to be 75.5 ± 2.36 % by HPLC, 33.085 ± 0.95 % by MS. ANOVA analysis is used to optimize the ratio between the components of liposome, which is found to be E6C2T2P2. Sucrose shows that minimal particle size changes after the freeze-dry study, while the weight ratio of sucrose to EGG PC is 30 : 1. Delivering puerarin, liposomal puerarin and proliposome of puerarin into HaCaT cell effectively inhibits inflammation and apoptosis induced by UVB. In addition, using low concentrations of liposome dosage can reach the same expectation as using high concentrations of puerarin. The role of UVB-induced apoptosis and inflammation was found that UVB up-regulation of MAPKs, PI3K/Akt, PKC, NF-κB and CPLA2 pathway via reactive oxygen species (ROS).
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