| Summary: | 碩士 === 中原大學 === 化學工程研究所 === 104 === Microalgae can convert carbon dioxide into biomass as a result of their photosynthetic activity. It is kind of potential raw material of biomass energy because it’s high growth rate and low land resource for cultivation. However, the most serious problem is that the microalgae culture medium is very expensive. The photosynthesis of microalgae can remove organic and inorganic nutrients in cassava wastewater before discharging to natural water environment. The wastewater was used as culture medium for Chlorella to culture microalgae and reduce the pollutant in the wastewater.
The single variable method was used to check whether the pre-culture medium, wastewater pretreatment, wastewater concentration and O.D. value is important for this research. And the response surface method was used to investigate the effect of illuminance, initial O.D. value and wastewater concentration. While 6000 lux of illuminance, 0.1 of initial O.D. value and 0.15X of wastewater concentration have the highest specific growth rate 2.05 day-1. 75% of COD, 78% of protein and 85% of TP can be removed under 6000 lux of illuminance, 2 of initial O.D. value and 0.15X of wastewater concentration after two days of culture.
For the application of microalgae, the cassava wastewater can used as pre-culture medium for Chlorella and culture under 12:12 light/dark cycle. Higher concentration of Chlorella and stable COD of wastewater can be achieved by using semi-continuous reactor under dilution rate between 0.1 to 0.15 during eight days of culture.
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