Kinetics of Biodegradation of Phenol and Sodium Salicylate by Pseudomonas putida.

• Main Authors: GUAN,LUN-CHEN, 程冠倫
• Other Authors: LIN,YEN-HUI
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/90067309477077239836

碩士 === 中臺科技大學 === 環境與安全衛生工程系碩士班 === 104 === Kinetic biodegradation of single phenol and sodium salicylate (SA) by Pseudomonas putida BCRC 14365 was studied at 30°C and pH 7.0. Experiments were conducted at different initial concentrations of phenol (0.33-3.86 mM) and SA (0.12-1.89 mM) in batch tests, respectively. Based on the specific growth rate of cells versus initial concentration on single phenol and SA, phenol had larger biodegradation rate than SA at a lower initial concentration (<0.7mM) in the single substrate batch tests. The magnitude of Ks value indicated that the P. putida has a slight stronger affinity to phenol in comparison with SA although the cell was adapted with SA. The Ki value revealed that P. putida cells is less sensitive to SA inhibition (Ki =4.98 mM) than phenol (Ki =1.211 mM) at a higher substrate level. Experimental results for binary substrates in batch tests show that the addition of SA to phenol did not significantly affect the time required for complete biodegradation of phenol. However, the presence of a small amount of phenol could accelerate the complete biodegradation of SA. Removal efficiencies for phenol and SA were approximately 98% at a steady-state condition in the packed-bed bioreactor. The model predicted the experimental results of phenol and SA as well as the growth of suspended cells fairly well for binary substrates in batch and packed-bed bioreactors. The structural approaches of modeling and experiments in this study can be used as tools to control and optimize the reactors performance of simultaneous biodegradation of mixed substrates in wastewater.