| Summary: | 博士 === 中山醫學大學 === 口腔科學研究所 === 104 === This study investigates the physicochemical and biological effects of natural medicines on the calcium silicate (CS) composite of bone cells using human dental pulp cell. CS is an osteoconductive and bioactive material. Hinokitiol is a natural material and it has antibacterial and anti-inflammatory effects. Xu Duan (XD) has been used in Traditional Chinese Medicine for hundreds of years as an anti-osteoporosis, tonic and anti-aging agent for the therapy of low back pain, traumatic hematoma, threatened abortion and bone fractures. For this research we have a series of CS composites with different ratios of Hinokitiol and XD were prepared to make new bioactive and biodegradable biocomposites for bone repair. Formation of bone-like apatite, the diametral tensile strength, and weight loss of composites were considered before and after immersion in simulated body fluid (SBF). In addition, we also examined the effects of Hinokitiol and XD released from CS composites and in vitro human dental pulp cell (hDPCs) and studied its behavior. The results show the hinokitiol-modified on CS cements had better antibacterial and anti-inflammatory abilities and cytocompatibility than CS cements. Otherwise, the hinokitiol-modified CS cements had suitable setting times and better odontoblastic potential of hDPCs. The XD on CS composite after immersion in SBF, the microstructure image showed a dense bone-like apatite layer covered on the CS/XD composites. In vitro cell experiments shows that the XD- rich composites promote human dental pulp cells (hDPCs) proliferation and differentiation. However, when the XD quantity in the composite is more than 5 %, the amount of cells and osteogenesis protein of hDPCs were stimulated by XD released from CS composites. In our study, hinokitiol-modified CS cements not only inhibited the expression level of inflammatory cytokines, but also had better cytocompatibility, antimicrobial properties and active ability of odontoblastic differentiation of hDPCs. The combination of XD in osteogenesis of CS gives strong reason to believe that these calcium-based composite cements may prove to be promising bone repair materials.
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