The role of Transglutaminase 2 in human lung cancer cell lines

• Main Authors: Bei-Syuan Syu, 許倍瑄
• Other Authors: 許國堂
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/e85m96

碩士 === 中山醫學大學 === 醫學研究所 === 104 === Previously studies have shown that high expression levels of transglutaminase 2 (TG2) were detected in several types of cancers. Moreover, the most universal and diverse member of the TG family is the TG2, a multifunctional protein. Since the leading cause of deaths in Taiwan (2014) were trachea, bronchus and lung cancers, we aim to investigate the role of TG2 in lung cancer cell lines (A549 and H1299 cells) to uncover its significance. The expression of TG2 in lung cancer cell lines was detected by real-time PCR, ELISA and western blotting. Down-regulation of TG2 in A549 and H1299 cells were achieved by transfecting cells with siNC or siTG2 RNA, and its effect on cell survival was evaluated. Our results showed that A549 cell has the highest expression level of TG2 as compared with H1299, H1355 and H460 cells. Inhibition of TG2 in A549 by RNA interference reduced colony formation and induced apoptosis and autophagy. Regulation of autophagy may be related to the AKT pathway. In addition, down-regulation of TG2 for 48 h and 72 h by siRNA in H1299 cell showed increased expression of the apoptosis marker, c-PARP and the autophagy marker, LC3A-Ⅱ by western blotting. Interestingly, H1299 cell transfected with siTG2 RNA failed to reduce levels of p-AKT. These results suggest that TG2 may play a protective role in A549 cell. In order to clarify the role of TG2 in lung cancer cell lines, the relationship between autophagy and apoptosis need to be further investigated in the future.