| Summary: | 碩士 === 中山醫學大學 === 營養學研究所 === 104 === Resveratrol (Res) is one kind of polyphenols, found in grape skins, red wine. Resveratrol has several biological activities, such as cardioprotective, anti-cancer, anti-oxidant and anti-inflammatory. Oxyresveratrol (oxy-Res) is a nature ingredient in mulberry (Morus alba L.) whereas acetyl-resveratrol (ac-Res) is a synthetic resveratrol. Res as an indirect sirtuins activator may work as a calorie restriction mimetic (CRM) to extend lifespan of organisms. However, the reports related to whether Res extended the lifespan of animal cells are not consistent. Our previous studies confirmed that calorie restriction could extend the lifespan of human Hs68 cells; however, whether Res, oxy-Res and ac-Res have abilities to extend the lifespan Hs68 cells is not clear. In addition, some researchers found that polyphenols in the medium was easily oxidized to produce hydrogen peroxide, thereby affecting the results of cell experiments; however, whether Res, oxy-Res and ac-Res were oxidatively degraded in the medium is also unclear. In this study, we used the high-performance liquid chromatography to analyze Res level in DMEM. The lifespan of Hs68 and IMR90 cells were measured as the cumulated growth curve of population doubling levels. The results showed that Res, oxy-Res and ac-Res in medium containing 10% FBS were not degraded. The ac-Res in the medium was shown to be hydrolyzed into Res but without any oxidative degradation. Importantly, we found that Res, oxy-Res and ac-Res had no ability to extend the lifespan of Hs68 cells. On the contrary, a high concentration of Res, oxy-Res and ac-Res will shorten the lifespan of cells. In addition, we found that physiological concentrations of Res did not inhibit the shortening of the cell lifespan induced by tert-butyl hydroxide due to oxidative stress. Although the results of this study do not support the Res as CRM, but Res may still be converted to other metabolites to having CRM effect, which needs further research. In addition, we demonstrate that pH value is the main factor for the Res degradation in the medium. We also found new products degraded from Res in NaHCO3 solution, which merited further investigations on their health functionalities.
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