The association between the 3954 polymorphism of interleukin-1β and colorectal cancer

• Main Authors: Jia-Rong Li, 李佳蓉
• Other Authors: 許立松
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/79333998153858816884

碩士 === 中山醫學大學 === 生化微生物免疫研究所 === 104 === Colorectal cancer (CRC) is the third most common malignancy worldwide. It has been proposed that risk factors for colorectal cancer include increased age, gender (men are more predisposed to the development of colorectal cancer), the presence of inflammatory bowel disease, certain hereditary conditions and a family history of colorectal cancer. The interleukin1β (IL-1β) protein has many pleiotropic biological functions including the mediation of inflammation by enhancing the movement of inflammatory cells from the blood to inflamed tissues and the regulation of extracellular matrix degradation. Reports indicate that single nucleotide polymorphism (SNP) is associated with cancers and diseases. We studied the association of the IL-1β-3954 C/T polymorphism and CRC in Taiwan population. Using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), the allele frequency of the IL-1β polymorphism was investigated in 74 patients with colorectal cancer and 72 control. It shows that polymorphism of IL-1β-3954 in between CRC onset patients and controls are significant difference (p<0.001). In addition, the frequencies of IL-1β-3954 homozygous T/T allele and C/T+C/C genotype in CRC group was not associated with stage (p=0.232)、tumor size (p=0.507)、 lymph node metastasis (p=0.142)、distant metastasis (p=0.265). Our study presents preliminary but intriguing data suggesting that IL-1β3954 may be associated with CRC onset in Taiwan region.