Study on the role of Metastasis Associated Protein-2 in Human Renal Cancer Cells

• Main Authors: Chang-Fang Yeh, 葉靜芳
• Other Authors: 謝逸憲
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/42812354023430081962

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 104 === MTA2 is a critical composition of the Mi2/Nucleosome remodeling and histone deacetylase (NuRD) complex, which contributed to the epigenetic silencing genes. More and more studies demonstrated MTA2 was associated with tumor cells proliferation, migration and invasion. In the previous studies it is shown MTA2 highly expressed in several tumors, including hepatocellular carcinoma, gastric cancer, glioma and breast cancer. However, the role of MTA2 of renal cell carcinoma is still unclear. Therefore, the aim of the study was to clear the biological function of MTA2 in renal cell carcinoma cells. At first, the renal carcinoma tissue array by immunohistochemistry staining suggest the MTA2 levels were positively associated with tumor grade, The MTA2 protein in four renal carcinoma cells (A498, 786-O, Achn and Caki-1) were higher expressed than in normal renal tubular HK2 cells. Following, the 786-O cells transfected by MTA2 shRNA was used for biological function investigation. MTT assay and Propidium iodide staining assay indicated knockdown of MTA2 had no effect on cells growth rate and cell cycle distribution, but inhibited the migration and invasion capacity of 786-O cells. In addition, knockdown of MTA2 in 786-O cells inhibited the protein expression of MMP-1 and MMP-9, but not MMP-2 protein. Moreover, knockdown of MTA2 might down-regulate ERK phosphoylation to inhibit cells migration and invasion. Based on the above evidence, MTA2 is important for renal cell carcinoma migration and invasion, and provides a potential target for clinical cancer metastasis research, and may open interesting perspectives to the strategy for human renal cell carcinoma therapy.