| Summary: | 碩士 === 嘉南藥理大學 === 保健營養系 === 104 === Background and purpose: Cisplatin (CP) is a very wide range of chemotherapeutic drugs, mainly used in the treatment of head and neck cancer, esophageal cancer, genital cancer, cervical cancer and other cancers. But also cause many side effects, such as: neurotoxicity, ototoxicity, renal toxicity, and nausea and vomiting, especially as the main limiting renal toxicity in clinical use. In this study, a single injection of CP cause acute renal failure (ARF), to Gynura formosana (G) immortality as a preventive medication, to assess whether the kidneys have a protective effect in this mode. Methods: Nine-week-old C57BL/6 strain male mice were divided into 4 groups according to different samples after 1 week of acclimation. Control group (C, administered playing saline 10mL/kg); treated group (CP, intraperitoneal injection of CP 20 mg/kg); sample groups were administered with aqueous extract 1 g/kg (GH) or 0.5 g/kg (GL) body weight. The mice were tube fed with aqueous extract or saline daily once for three weeks, then a single CP injection was given except C group. After 72 hours of CP injection, mice were sacrificed. Results: Gynura formosana extract for three hours has the highest levels of total flavonoids. Caffeic acid and Rutin was found by HPLC separation, howerver, no significant peak of Gallic acid and Quecertin was observed in the extract. Relative tissue weights showed no significant differences between the groups in heart, testes, EP, RE. Levels of serum BUN and LDH in CP group were significantly higher than that in group C (P <0.05). GPX activity of kidney in GH group was significantly higher than that in CP group (P <0.05). The acute decline of kidney GPX activity induced by CP injection could be restored with high dose of Gynura formosana extract.
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