Effect of lactic acid bacteria from fermented mustard on intestinal microflora distribution and antiproliferative action of phytochemicals on colorectal adenocarcinoma SW480 cells

• Main Authors: Siao,Zhen-Ting, 蕭甄庭
• Other Authors: Chen,Shih-Ying
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/v5ajew

碩士 === 嘉南藥理大學 === 保健營養系 === 104 === The purpose of this study was to evaluate the probiotic properties of lactic acid bacteria (B0091) strain isolated from fermented mustard in vivo. Male Wistar rats (10-week-old) were randomly divided into three experiments groups (7 rats/group) that were high dose (1× 1011 CFU/kg bw), median dose (2× 1010 CFU/kg bw) and low dose (4× 109 CFU/kg bw) of B0091 were gavage for 5 consecutive weeks. Furthermore, the positive control was orally-administered L.casei (1× 1011 CFU/kg bw), the control was treatment with the normal saline instead of sample administration. All animals were acclimatized for 1 week prior to use. The body weight and food intake were recorded daily. Fecal and fecal of cecum samples were collected to determine number of beneficial bacteria bifidobacterium and harmful bacteria Clostridium perfringens by classical plate count and by PCR amplification. Strain B0091 had a significant improve intestinal microflora distribution compared to the control. In addition, the villus height (vh), crypt depth (cd) and vh/cd ratio of rats’ jejunum were determined. The vh and vh/cd ratio were increased, which may suggest strain B0091 modulated intestinal mucin composition. Collectively, these results obtained from in vivo experiments positive effect of strain B0091 on antibacterial activity and thereby leading to improvement of intestinal health. Colorectal carcinoma is the third leading cause of cancer-related death in Taiwan. In order to improve the prognosis, chemotherapy is often used in a variety of clinical situations. The toxicity of these chemotherapeutic agents to normal tissues has been one of the major obstacles to successful cancer chemotherapy. Therefore, combined treatments with adjuvant therapy are often used not only to enhance the treatment effect, but also to reduce the toxicity of these drugs. 5-fluorouracil (5-FU) has been the major chemotherapeutic agent for treating colorectal carcinoma; however, response rates have been around 20–35% with median overall survival no more than 1 year. So finding anti-cancer compounds with high nutraceutic effect which can be used in combination with existing drugs may provide an important way forward in the treatment of colorectal carcinoma. Based on our researches, fractionation in ethanolic extracts from Polygonum cuspidatum root (PcE(H)EA) , Supernatant from the 95% ethanolic extracts of Morinda citrifolia leafexhibit (LES) , and water extracts of sweet orange (Citrus sinensis) peel (WESP) exhibit various and higher contents in polyphenols, and reveal that these compounds play a part in antioxidative and anti-inflammatory activity in vitro. Hence, PcE(H)EA, LES, WESP and its active components are worth further research in purification, and deeply investigation for anti-cancer effects. This study aim to investigate the inhibitive effects of active components from PcE(H)EA, LES, WESP combined with 5-FU on colorectal carcinoma (SW480) cells in vitro. The inhibition effects of cell proliferation of test sample and 5-FU, either in combination or on their own, are will be observed. According to the inhibition rate of cell viability, half maximal inhibitory concentration and combination effect, results in the order of efficacy showed that resveratrol plus emodin at concentration ratio of 1：4 (w/w)＞Emodin＞PcE(H)EA＞Hesperetin＞Hesperidin＞WESP＞Resveratrol＞F1＞Rutin＞LES with 5-FU in SW480 cells. This is a very practicable strategy of adjuvant therapy—they might avoid the rapid development of resistance that occurs to drugs that target cancer cells.