Hypouricemic effect of Gynura formosana extract using long-time water boiling

• Main Authors: ZHANG, JIAN-GUO, 張健國
• Other Authors: SHAW, HUEY-MEI
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/83918016125192401280

碩士 === 嘉南藥理大學 === 保健營養系 === 104 === Background and Purpose: Hyperuricemia has become a risk foctor of kidney disease, hypertension and other chronic diseases. Therefore the control of plasma uric acid levels is important. The purpose of our study, is to investigate whether the hot water extract of Gynura formosana as shows the hypouricemic effect in mice. Methods: 35 three-week-old ICR male mice, after a one week adaptation, were divided into the following 5 groups: C group (normal control group), P group (hyperuricemic modle), A group (Allopurinol treatment group), GL and GH group was tube fed with Gynura formosana extract 100 mg/kg BW, 500 mg/kg BW respectively, group C was injected with 0.5% CMC solution, the other groups are intraperitoneal injectied with PO 250 mg/kg, daily for continous 7 days.Group C and P were tube fed with saline, group A was tube fed with Allopurinol (10 mg/kg) daily once, group GL and GH were tube fed with the test sample. After animal sacrifice, plasma uric acid concentration, urea nitrogen (BUN), creatinine were assayed, The enzyme activtites of xanthine oxidase (XO) and xanthine dehydrogenase (XDH), catalase, Glutathione Peroxidase, superoxide dismutase in liver were also measered. We also measured the levels of lipid peroxidation index (TBARS) in liver. Results: Plasma uric acid levels in P group were significantly higher than other groups, Allopurinol medication and GF exteact feeding were significantly decreased the plasma uric acid levels. Gynura formosana water extact also significantly decreased, the XDH enzyme activity. Liver GSH contents in GL, GH group were significantly lower than that in P group. The activities of CAT, SOD, GPx in GH group were significantly lower than that in P group. Conclusion: The results indicate that Gynura formosana extract shows the hypouricemic effect which could be due to th inhibition of liver XDH activity.