笑氣對大鼠血腦屏障通透性的影響

• Main Authors: Jen-Kuo Hsu, 徐仁國
• Other Authors: 賴威達
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/34755793377854180165

碩士 === 中國醫藥大學 === 臨床醫學研究所碩士班 === 104 === BACKGROUND The blood-brain barrier plays an important role of maintaining the function of central nervous system. The integrity of blood-brain barrier is closely related to the prognosis of the patient especially in brain surgery. Inhalational anesthetics were frequently used during operation. Some inhalational anesthetics have variable degree of influence on blood-brain barrier permeability. The goal of this study is to uncover the impact on blood-brain barrier permeability of the nitrous oxide-an inhalational anesthetic. OBJECTIVE Evans Blue dye is an inert tracer frequently used to evaluate the permeability to high molecular weight substance of blood-brain barrier . After being injected with Evans Blue dye into the tail vein, the rats were then put into a translucent plastic box filled with different concentrations of nitrous oxide. We utilize spectrophotometry to detect the absorbance of residual Evans Blue dye in different brain regions to see if the nitrous oxide would alter the permeability of blood-brain barrier. METHODS The rats were allocated to five groups (Control、20%N2O、40%N2O、80%N2O、20%Carbogen). Isoflurane was used to anesthetize the rats. Evans Blue dye was injected into the tail vein of rats. Thereafter, the rats were put into a translucent plastic box filled with different concentrations of nitrous oxide according to its group for 90 minutes. Blood sample were collected from femoral artery after inhalation. After 10 minutes perfusion of normal saline, the brain was harvested for collecting samples from different brain region. A piece of liver tissue was also collected. Evans Blue dye contained in blood、liver and brain tissue was retrieved using trichloroacetic acid. Spectrophotometry was utilized to detect the absorbance of Evans Blue dye of samples. After converting the absorbance value to real content of Evans Blue dye of tissue, the content of Evans Blue dye of experimental groups was compared using statistics. RESULTS There was no significant difference of Evans Blue dye content of different brain regions among nitrous oxide groups, and there was also no significant difference when comparing the nitrous oxide groups and control group. CONCLUSIONS In conclusion, up to 80% nitrous oxide up don’t change the blood-brain barrier permeability to Evans Blue dye in rats under normal brain physiology.