| Summary: | 碩士 === 中華大學 === 生物資訊學系 === 104 === The basidiomycete white rot fungus Ganoderma lucidum (Leyss ex fr) Karst (Lingzhi; Reishi) has been used for long time in East Asia. Modern research suggests that the main harmacological components are Ganoderma polysaccharides and Ganoderma triterpenoids. Ganoderma triterpenoids possessed many biomedical effects such as anti-tumor, liver, antihypertensive, cholesterol-lowering, anti-HIV and anti-histamine. Although a lot of studies were done about Ganoderma, the focus is on the biomedical activities of their extracts. However, few studies were done on the synthesizing mechanism and biological pathways of Ganoderma triterpenoids. In 2012, two genomes of G. lucidum were sequenced by two research groups in China, which enable us to study the synthesizing mechanism of Ganoderma triterpenoids using comparative genomics. In this paper, possible genes involved in the synthesizing of Ganoderma triterpenoids were discovered using the KEGG, UniProt, and GenBank databases, and two G. lucidum genomes. Six P450 genes which may involve in the synthesizing of Ganoderma triterpenoids were also derived by comparing the expression profiles of the aerial mycelia of dikaryons and fruiting bodies. Polymerase chain reaction experiments were performed to verify the existence of these genes. Based on the experiment results, a putative pathway model for the synthesizing of Ganoderma triterpenoids is constructed.
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