| Summary: | 碩士 === 中華科技大學 === 健康科技研究所在職專班 === 104 === The purposes of this study were to (l) confirm the effect of truffle inoculation on the seedlings growth of Cyclobalanopsis glauca, (2) observe the competition of Scleroderma areolatum to the mycorrhizae formed either by Tuber formosanom or T. indicum and (3) compare the competition of S. areolatum to the truffle cultured on YMT agar medium.After seedlings of C. glauca were inoculated with T. formosanum and T. indicum, the height growth, root collar diameter, top dry weight and root dry weight were much more significantly stimulated than those of non-inoculated seedlings. The T/R ratio of both Tuber-inoculated seedlings was significantly lower than those of non-inoculated seedlings. S. areolatum could form typical white ectomycorrhizae when it was inoculated to one year’s old C. glauca seedlings mycorrhized either with T. formosanum or T. indicum two months later after inoculation. The other uninoculated rootlets could be infected by the rhizomorph extended from white ectomycorrhiza. The rhizomorph could also infect the Tuber-infected ectomycorrhizae and finally enveloped it. Therefore, the inoculation of S. areolatum could reduce or impede the mycorrhiza formation formed by T. formosanum or T. indicum. The Tuber- mycorrhized C. glauca seedlings inoculated with S. areolatum two months later could also stimulate seedlings growth, as the seedlings were inoculated with truffle only.YMT agar medium was used to test the colony competition between T. formosanum and S. areolatum, T. formasanum and S. areolatum each other. The results showed that the colony growth of either T. formosanum or T. indicum grew much quicker than that of S. areolatum, and could impact the growth of S. areolarum. There was compete dominance for the colony growth of T. indicum, especially. The colony of T. indicum could cover the colony of S. areolatum at the end of the test. The colony of T. formosanum and S. areolatunn grew mixedly each other at the late stage. Neither the hyphal anastomoses of T. indicum and S. areolatum, nor the hyphal anastomoses of T. formosanum and S. areolatum were observed.
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