| Summary: | 碩士 === 長庚大學 === 醫學生物技術暨檢驗學系 === 104 === Obesity is considered to be a disease condition that associated numerous health problems, leading by low-grade inflammation and intestinal dysbiosis. The traditional medicinal mushroom, water extract of Ganoderma lucidum mycelium (WEGL), reduces body weight, inflammation, insulin resistance, and modulating the gut microbiota in mice fed with high-fat diet (HFD), the beneficial effects not only displaying in WEGL but also in high molecular (>300kDa) polysaccharides of G. lucidium (G1). However, the interaction between high molecular weight polysaccharide (>300kDa) isolated from G. lucidium (G1) and gut microbiota to reduce obesity is still unclear. Thus, we anaerobically culture feces from HFD-fed mice and chow-fed mice with high molecular weight polysaccharides (G1), and nine days later we separated bacteria and supernatant by high speeds centrifuge. The bacteria and supernatant are intra-gastric gavage everyday to HFD-fed mice. We show that bacteria, which are isolated from anaerobic culture HFD-fed mice feces and G1, have ability to reduce obesity, ameliorate inflammation cytokine expression and insulin resistance effect in HFD-fed mice.
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