Development of Biodegradable Hemostasic and Analgesic Nanofibrous Membranes for Oral Applications

• Main Authors: Tzu Chia Lee, 李子嘉
• Other Authors: S. J. Liu
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/r49x7r

碩士 === 長庚大學 === 機械工程學系 === 104 === Free gingival grafts have been used as a predictable and reproducible technique for increasing keratinised gingiva around natural teeth. However, the postoperative morbidity associated with an open secondary wound site leads to inconvenience caused by pain and post surgical bleeding. The goal of this study was to develop the biodegradable PLGA nanofibrous membranes loaded with lidocaine, epinephrine and collagen by electrospinning. The release characteristics of medications and collagen loaded PLGA nanofibers were also evaluated by the high-performance liquid chromatography (HPLC). Wounds at the palate donor site were created in rabbits for in vivo analysis, and the hemostasic and analgesic analysis were examined at two weeks after operation. The experimental results showed that the average diameter of electrospun nanofibers was 591 nm and the fibers exhibited hydrophilic and ductile characteristics. The results of HPLC analysis suggested that the drug-eluting nanofibers can provide sustained release of drugs for 27 days. Especially, it showed high drugs concentration at first three days. In addition, animal experiments proved that the membranes exhibit good hemostasis property. Biodegradable drugs loaded PLGA nanofibers displayed outstanding ability for wound repairs.