Development of Reflection-Based Optical Waveguide Particle Plasmon Resonance Biosensing Platforms

• Main Authors: JIA-YU SANG, 宋家裕
• Other Authors: Lai-Kwan Chau
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/68801620211485505316

碩士 === 國立中正大學 === 化學暨生物化學研究所 === 104 === The objectives of this work are to develop two novel multiplex chemical and biochemical sensing platforms, namly a reflection-based tubular waveguide particle plasmon resonance (RTW-PPR) biosensing platform, and a reflection-based fiber optic particle plasmon resonance (RFO-PPR) biosensing platform. The principle of inventions are based on measuring the light intensity after consecutive total internal reflections (TIRs) along a noble metal nanoparticles-modified waveguide (tube or optical fiber), wherein the evanescent wave excites the particle plasmon resonance of the nanoparticles at the reflection interface. When a noble metal nanoparticle is influenced by the change of the refractive index on its surrounding environment, its particle plasmon resonance condition will change. This phenomenon can be used as the basis of chemical and biological sensing. In the first part ：we used Poly(methyl methacrylate) PMMA as waveguide material to form a tubular waveguide and utilized 3-mercaptopropylsilatrane (MPS) to reduce the modification time. A variety of experiments were carried out to validate the sensitivity and refractive index resolution of the sensing platform. Using different weight percent of sucrose in pure water as samples, a refractive index resolution of 4.34×10-5 RIU and a sensor sensitivity of 5.39 RIU-1 have been achieved by the platform. In the biochemical detection experiments, OVA was used to functionalize the gold nanoparticle in order to detect anti-OVA. Results show that the calibration curve is linear (R2>0.99) and the limit of detection (LOD) is about 4.64×10-6 g/mL (3.09×10-8 M). In the second part：the RFO-PPR platform has achieved the absorbance sensitivity of 4.83 AU/RIU-1 and the sensor resolution of 4.6×10-5 RIU by using gold nanospheres as the sensing element. By the similar configuration, but using gold nanorods as the sensing element, the absorbance sensitivity of 3.81 AU/RIU-1 and the sensor resolution of 3.7×10-5 RIU have been achieved. In the biochemical detection experiments, DNP was used to functionalize the gold nanorods in order to detect anti-DNP antibody. Results show that the calibration curve is linear (correlation coefficient >0.99) and the detection limit is less than 3.88×10-10 M.