Differentiation of induced pluripotent stem cells in activin A and bone morphogenetic protein 4 grafted with poly(vinylalcohol)/alginate/gelatin self-assembled hydrogel scaffold co-culture with retinotic acid in solid lipid nanoparticles

• Main Authors: Lee, I-Hsuan, 李翊瑄
• Other Authors: Kuo, Yung-Chih
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/61161758987683049886

碩士 === 國立中正大學 === 化學工程研究所 === 104 === In this research, natural polymer poly(vinyl alcohol) (PVA),alginate (Alg), and gelatin (Gel) are chosen as main components of substrate and than photocrosslinked to produce numerous 3D microgels. Repeat unit microgels self-assemble by interface force in oil-water two phase systems. An increase in the weight percentages of PVA and Alg increase the swelling ratio and porosity of hydrogel scaffolds system. The surfaces of hydrogel scaffolds system was modified with activin A and mouse bone morphogenetic protein 4 (BMP4) treated with retinoic acid (RA)-loaded solid lipid nanoparticles (SLNs) guides the differentiation of induced pluripotent stem cells (iPSCs) toward endodermic and pancreatic cells. An increase in the weight percentages of Gel increase the entrapment effiency and viability of iPSCs. The flow-cytometric analysis indicated that the percentages of SOX17 increased 35.00% of activin A-BMP4-grafted hydrogel scaffolds (ractivin A/(activin A+BMP4) = 75%) compared to hydrogel scaffolds without grafted activin A and BMP4. The flow-cytometric analysis indicated that the percentages of PDX1 increased 21.90% of activin A-BMP4-grafted hydrogel scaffolds culture with RA in SLNs (0.5 mg/mL) compared to hydrogel scaffolds culture without RA in SLNs.