Fermentation strategy of producing glucosamine using low cost medium

• Main Authors: Dian-Siou Li, 李典修
• Other Authors: Ho-Shing Wu
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/k5qdnj

碩士 === 元智大學 === 化學工程與材料科學學系 === 103 === Glucosamine is an amino monosaccharide. It is an important part of mucopolysaccharides and chitin. Glucosamine is a human joint lubricant and it can repair and rebuild damaged cartilage and relieve osteoarthritis disease. Use shrimp shells to produce glucosamine which faces marine pollution and fish catches and. Using fungi to produce could overcome these problem. This work investigates the production of glucosamine hydrochloride, and focus on the reduction of production costs, using a various ingredients of medium culture fermentation in a 250 mL flasks and 5-liter fermenter. In this study, isolation and purification methods were investigated to obtain glucosamine hydrochloride. Aspergillus sydowii was used for production of glucosamine fungi. This study attempts to use different medium that cultivated different generation fungi. The fermentation conditions such as organic and inorganic nitrogen source and carbon source, fungi growth generation and kind of salt are facilitated to find obtain the optimal production of glucosamine. The experimental design in the study used response surface methodology. To obtain the optimal fermentation conditions for the molasses 40.12 mL/L, corn steep liquor 49.89 mL/L. Glucosamine hydrochloride was obtained 1.36 g / L, significantly reducing production costs. If used sugar and peptone medium working in volume of 3 L fermenter at temperature 30 oC, 50% dissolved oxygen, for 5 days at 500 rpm, glucosamine hydrochloride was obtained 5.11 g / L, and productivity of 30.41 mg / L • h and biomass 32.1 g / L. Used sugar and corn steep liquid and phosphate diamine as main composite medium, glucosamine hydrochloride can be obtained 2.01 g / L, productivity of 11.96 mg / L • h and biomass 15.13 g / L. In the method of separation, in the study, to increase the purity and recovery, using ethanol to produce a crystalline precipitate can be obtained with a purity of 75% glucosamine hydrochloride, the recovery was 58%. The study also used patent analysis to look for the future development of this area with the current study focused direction.