Chitosan graft copolymerized with monomers containing quarternary ammonium group as antimicrobial wound dressing

• Main Authors: Po-Han Chen, 陳柏翰
• Other Authors: Kuo-Yu Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/6863q4

碩士 === 國立雲林科技大學 === 化學工程與材料工程系 === 103 === Chitosan is widely used in biomedical applications due to its antibacterial property. However, the antibacterial activity of chitosan significantly reducesat physiological pH (7.4). In the study, [2-(acryloyloxy)ethyl]trimethylammonium chloride (AETMAC) was grafted onto chitosan using ammonium sulfate (APS) as a redox initiator to improve its antibacterial activity in neutralpH. The effects of APS and AETMAC concentrations and reaction temperature on graftingpercentage and grafting efficiency were studied.Theincorporation of AETMAC into chitosan chains was confirmed by Fourier transforminfrared (FTIR) spectroscopy.The effects of grafting on thedegree of crystallinity, solubility, water absorbance ratio, water vapor transmissionrate (WVTR) and antibacterial activity of graft copolymer (CS-g-PAETMAC) membraneswere investigated. The results showed that the graftingpercentage and grafting efficiency increased with increasing the concentration of APS and reaction temperature. Moreover, the grafting efficiency decreased with increasing the concentration of AETMAC. CS-g-PAETMAC had a higher graftingpercentage when the concentration of AETMAC was 0.15 M. FTIR spectra confirmed the formation of graft copolymer and thefact by increasing the amount of AETMAC in the feed mixture, the amount ofincorporation AETMAC in the graft copolymers increased.X-Ray diffraction exhibited that the grafting of AETMAC disturbed the crystalline structure of chitosan. Additionally, the CS-g-PAETMAC membrane has a higher solubility in pH 5.0 and pH 7.4 solutions, water absorbance ratio and WVTR than pure chitosan membrane. Moreover, the higher the concentration of AETMAC, the higher were solubility, water absorbance ratio and WVTR.Finally, the antibacterial activity of the chitosan membranes wasperformed by inhibition zone method against Staphylococcus aureus. It demonstrated a distinct inhibition zoneadjacent to CS-g-PAETMAC membrane when the concentration of AETMAC was higher than 0.15 M. However, it was not observed around the pure chitosan membrane. Therefore, the CS-g-PAETMACmembranehas the potential for wound-dressing application.