| Summary: | 碩士 === 國立陽明大學 === 口腔生物研究所 === 103 === Oral squamous cell carcinoma (OSCC) is an oral cancer. There will be about 600,000 cases per year occur in the world, of which only 40 to 50 percent of the five-year survival rate of patients. Smoking, drinking, chewing betel nut, human papilloma virus infection are the reason which may cause oral cancer. Oral cancer and DNA damage repair gene mutated are highly correlated, many research showed that ARID1A mutations are also involved. ARID1A is a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. ARID1A play an important role in ovarian cancer, endometrial cancer and gastric cancer, but the mechanism of oral cancer is still not very clear. So we would like to investigate the molecular mechanisms related to oral cancer and ARID1A. I established SAS and OECM1 two kinds knockdown ARID1A oral cancer stable clones to confirmed EMT-associated protein then found cancer stem cell-associated proteins are upregulation. Also found ARID1A may regulate cell migration, invasion and anchorage. Furthermore, used flow cytometry to test the cancer stem cell related enzyme then found that are increased. Also used immunohistochemistry to observed the mouse oral cancer tissue and found it would be decreased with the number of weeks. It suggests, ARID1A may regulate the characteristics of cancer stem cell and the degree of cancer malignancy.
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