| Summary: | 碩士 === 國立陽明大學 === 生物醫學工程學系 === 103 === Stroke is a cerebrovascular disease that is caused by the blockage of blood vessels, which can result in permanent neurological damage or death. Previous studies have shown that 12-(3-adamantan-1-yl-ureido) dodecanoic acid (AUDA) can lead to protection mechanisms of the brain, heart and kidney. However, the exact mechanism of this phenomenon still remains unclear. Though there has been much research focused on stroke, the treatment options in the acute phase of stroke are still limited.
Normally neural activity is accompanied by changes in the local oxygenation and blood flow. When the neurons were stimulated, oxygen and energy is needed to support the reaction. The relationship between neural activity, oxygen metabolism, and hemodynamic can be studied by variable imaging techniques. Simultaneously imaging the changes of blood flow, blood volume, and oxygenation in tissue is important for basic research in biological science, clinical diagnosis, and therapeutic applications.
This study demonstrates optical imaging method for in vivo imaging of functional neurovascular activation during the stroke, treated by AUDA. Laser Speckle Contrast Imaging (LSCI) is an easy method to determine the relative blood flow, so we propose to use a synchronized two wavelength imaging system which combines laser speckle contrast imaging with Intrinsic optical signals imaging(IOSI). This system calculated LSCI data from the reflectance of the laser on the surface and records the oxygenation data from laser absorption in hemoglobin simultaneously. Based on this system, we also images changes in vessel density and try to separates arteries and veins in order to get more information by using the same raw data.
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