Discover circulating microRNA as potential biomarkers from the plasma of coronary artery disease patients and investigate their regulatory mechanism

• Main Authors: Hsiu-Yen Ma, 馬秀燕
• Other Authors: Hsei-Wei Wang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/02659853019034828464

碩士 === 國立陽明大學 === 微生物及免疫學研究所 === 103 === Endothelial progenitor cells (EPCs) has been found to have the potential to promote vessel repair and their dysfunction has been reported in coronary artery disease (CAD) patients .Many studies show that there are lots of miRNAs up regulated in CAD EPCs and they will inhibit angiogenesis. In our studies, we find that treating healthy EPCs with CAD EPCs- derived cultured medium and cultured medium exosomes can inhibit EPCs ability of tube formation and migration. We guess miRNAs in EPCs will release to culture medium and go into EPCs to regulate cells functions and they also could release to plasma form EPCs. Coronary artery disease is increasing in recent years. Early detecting CAD can prevent myocardial infarction and sudden cardiac death and therefore we want to find out biomarkers to early detect CAD. Besides early detect CAD, biomarkers also can help to assess vascular status after drug therapy. Studies show miRNAs have many properties to become good biomarkers ; therefore, we search miRNAs form plasma. We find that miR-146a-5p、miR-146b-5p、NM-4791、miR-99b-5p、miR-127-3p、miR-210-3p、miR-26a-5p are up-regulated in CAD plasma and they may become CAD potential biomarkers. To investigate regulatory mechanism of these miRNAs, we find miR-210-3p、miR-146b-5p、miR-26a-5p、miR-127-3p could target mitochondria related genes .Therefore, we test mitochondria function in healthy and CAD EPCs and we find that there are no difference between healthy and CAD EPCs. CAD is ischemic heart disease; therefore, cells could be in hypoxia environment. Thus, we treat EPCs with hypoxia and test mitochondria function. Results show that CAD EPCs have higher basal respiration, maximal respiration and ATP Production after treat them with hypoxia. Studies show that peripheral blood mononuclear cells of diabetes mellitus patients have the same phenomenon and it is a direction for further investigation.