Fabrication of tissue engineered oral mucosa equivalent in xenogeneic serum free environment

• Main Authors: Hung-Chuang Huang, 黃弘昌
• Other Authors: Wen-Liang Lo
• Format: Others
• Language: en_US
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/85522387770619505111

碩士 === 國立陽明大學 === 牙醫學系 === 103 === We have successfully cultured primary human oral keratinocyte without the supplement of xenogeneic serum or feeder cell layer, namely, a “clean” culture environment that is preferred for the future auto-transplantation. However, the cultured epithelial sheets, without underlying dermal-mimic component, are difficult to manage, easy to tear and tending to contract after application in vivo. In recent years, tissue engineering has become an emerging new field and provide three-dimensional culture technique for human oral mucosa. Generally speaking, human oral mucosa equivalent can be fabricated in vitro by cultured oral keratinocyte with or without fibroblasts on biocompatible scaffold. In the current work, the cross-linked collagen scaffold from rat tail (group 1) and the only human de-epidermized dermis (DED) approved by Taiwan Ministry of Health and Welfare (MOHW)(group 2) were used to try to fabricate oral mucosa equivalent. In group 1, we rarely can found cell attachment but with some cell nests infiltration into the collagen sponge. In group 2, cell attachment is also rare. We thought the failure of 3-D cultured oral mucosa equivalent in group 1 might be atrributed to the relative large pore size of the collagen sponge. And in group 2, it may be related to the preservatives of the DED (OrACELL®).