| Summary: | 碩士 === 國立陽明大學 === 公共衛生研究所 === 103 === Background
Smoking is a major risk factor for many diseases, for instance, cancer, cerebrovascular diseases, respiratory and other diseases, which are all in the top ten causes of death in Taiwan. Secretion of nicotine in cigarettes can stimulate the dopamine and serotonin in the brain, and serotonin affected by the sensitivity. As a result, it causes the smoking addiction. Previous studies found that serotonin receptor genes polymorphisms are associated with smoking behavior in European, but there was lack of evidence in Chinese. The goal of this study is to evaluate 5HT2A gene, 5HT3A gene and 5HT3B gene polymorphisms of serotonin receptors on the relationship with smoking behaviors.
Methods
We conducted the matched case-control method for gender, age ± 5 years old by 1: 1 matching in the study design. The study was including two parts: The first part was the physical checkup program from New Taipei City community from January 2011 to March 2012, which was included 1593 subjects in total. We selected 357 pairs to subsequent analysis. The second part subjects were the participants of physical checkup from the Taipei Veterans General Hospital from April 2014 to October 2014, which was included 251 pairs. We analyzed those data after matching .We selected four polymorphisms on three genes (5HT2A: rs6313, 5HT3A: rs33940208, rs1176713 and 5HT3B: rs1176744) and performed genotyping using the TaqMan Assay.
In this study, we explored the association between genotype and smoking status using the conditional logistic regression model to do the analysis. Besides, we explored the association between genotype and smoking addiction (Cigarette per day and smoking cessation with logistic regression model. In addition, we explored the relationship between haplotypes and smoking status, smoking addiction and smoking cessation with PHASE, version 2.1.
Results
In community data, we found that rs1176713 of the 5HT3A gene with TC and CC genotype was easier smoking than TT genotype (ever smokers verse never smokers, OR=1.41, 95% C.I.=1.04-1.91). In addition, the rs1176744 of the 5HT3B gene with TG and GG genotype was less addictive than TT genotype (OR=0.42, 95% C.I.=0.19-0.93).
In the hospital data, the rs1176744 of the 5HT3B gene with TG genotype was less addictive than TT genotype (OR=0.37, 95% C.I.=0.14-0.97). In addition, rs1176713 of the 5HT3A gene with TT genotype was more difficult to smoking cessation than CC genotype (OR=2.96, 95% C.I.=1.02-8.56). Besides, the CC haplotype carrier by 5HT3A gene rs1176713 and rs33940208 had more difficult to smoking cessation than non-carrier (OR=2.02, 95% C.I.=1.21-3.39).
Conclusions
In the present study, we found that the rs1176713 of the 5HT3A gene with C allele was association with smoking status and smoking cessation. In addition, the rs1176744 polymorphism of the 5HT3B gene with TG genotype would influence smoking addiction. On the other hand, the haplotype of 5HT3A gene is an important role that would influence smoking cessation.
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