Response to copper excess in Arabidopsis thaliana and response to different concentrations of nickel in Brassica rapa.

• Main Authors: Wang, Chih-Wei, 汪志威
• Other Authors: Tseng, Mei-Hwei
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/24578316358104679500

碩士 === 臺北市立大學 === 應用物理暨化學系 === 103 === Copper and nickel are essential trace elements for plant growth, but at excess level, they become phytotoxic. This experiment used Arabidopsis thaliana and Brassica rapa as experimental material to investigate the impact of heavy metal on plants. To understand the impact of excess copper ions on thaliana and the impact of different concentration nickel ions on growth and metabolite level of plants, we measured biomass, DMC%, lipid peroxidation, photosynthetic pigments, water-soluble protein, sulfur-metabolite SAM, SAH, GSH, GSSG, Met, Cys, sulfate, nitrate and copper content. Under excess copper ion environment, it was found that biomass decreased, DMC% increased, lipid peroxidation increased, photosynthetic pigments decreased, water-soluble protein decreased, SAM content increased in shoot but decreased in root, SAH content increased in root but decreased in flower stem, SAM/SAH ratio increased in shoot and flower stem but decreased in root, GSH content slightly increased in shoot and root, GSSG content increased in root and shoot, GSH/GSSG ratio decreased, Met content increased, Cys content increased in shoot and flower stem but decreased in root, Copper content increased in root and upper tissue, and iron content increased also in root. Under increased nickel ion environment B. rapa biomass decrease, DMC% increased, photosynthetic pigments decreased, sulfate decreased in shoot and nitrate decreased in root.