Application of ConA – Sepharose Chromatography on Partial Purification of Fructofuranosidase

• Main Authors: Meng-Lan Tung, 童夢蘭
• Other Authors: Dey-Chyi Sheu
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/97223535003297196880

碩士 === 大同大學 === 生物工程學系(所) === 103 === The intracellular neo-fructosyltransferase from the yeast Xanthophyllomyces dendrorhous BCRC 21346 catalyzes the conversion of sucrose into neofructooligosaccharides (neo-FOS).Neo-FOS comprises neokestose and eonystose. β-Fructofruanosidase from culture of Xanthophyllomyces dendrorhous BCRC 21346 can converte sucrose into neofructooligosaccharides (neo-FOS),which is an important prebiotic. Concanavalin A (Con A) is a lectin isolated from Canavalia ensiformis (Jack Bean). It specifically binds to mannose, glucose and glycoproteins containing mannose. Lectin is used as a tool in identification and measurement of glycoproteins. Concanavalin A(Con A) is the first lectin found, therefore it is most studied and best choice for modeling novel protocols. In order to develop a simple and rapid purification process for glucoproteins, Con A-Sepharose was used for the purification of fructofuranosidase from X. dendrorhous, because in asingle process more than hyndreds folds of purification could be achieved.