PROTECTIVE EFFECTS OF MIMOSA PUDICA AGAINST UVB-INDUCED DAMAGE OF HUMAN FIBROBLASTS

• Main Authors: Chia-Jung Chou, 周佳蓉
• Other Authors: Shiow-Ling Lee
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/4ec88p

碩士 === 大同大學 === 生物工程學系(所) === 103 === Ultraviolet radiation of skin resulted in oxidative stress, causing photoaging of skin. Recently the anti-photoaging property of herbs had attracted much attention. The protective effects of Mimosa pudica against UVB-induced damage of human fibroblasts CCD966SK was determined in this study. The results showed that 100 ?慊/ml of methanol extract of M. pudica did not reduce the cell viability, indicating low cytotoxicity of M. pudica to CCD966SK cells. A dose-dependent decrease in cell viability was found in UV radiated CCD966SK cells. However, cell viability and collagen formation increased in CCD966SK cells pretreated with increasing methanol and water extracts for 24 h, respectively, followed by UVB radiation. Methanol extracts of 100 ?慊/ml exhibited the maximum effect without statistically significant difference to ascorbic acid as a positive control. This demonstrated that M. pudica increased the viability and collagen production in UVB-exposed CCD966SK cells. A significant decrease in glutathione and increase in malondialdehyde were obtained in CCD966SK cells with UVB irradiation. Pretreatment of M. pudica also promoted glutathione and reduced malondialdehyde amounts in CCD966SK cells with UVB exposure. Methanol extract had significant better effect than water extract on the CCD966SK cells exposed at 25 and 50 mJ/cm2. These results indicated that M. pudica could reduce the UVB-induced damage in human dermal fibroblasts.