| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 103 === Among various nitrogen sources used in submerged cultures of Rhizopus oryzae for lactic production in this work, urea proved the best in terms of not only biomass formation but also lactic acid production. At urea concentrations lower than 0.6 g/L, large mycelial clumps formed. And at urea concentration higher than 0.8 g/L, small pellets were obtained. However, when 0.05 g/L urea were added daily, cultures exhibited floc-morphology and the highest lactic acid concentration of 84 g/L with the yield of 0.7 was obtained. Lactic acid production was affected by (1) the concentration and addition mode of urea, and (2) mycelia morphology.
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