| Summary: | 博士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 103 === Since titanium alloys have well biocompatibility and proper mechanical properties, they are widely used as orthopedic implants. However, there still remain many problems in clinical, and they lead to transplant failure, causing from incomplete cell attachment, incomplete osseointegration or bacterial infection. In order to solve the problems and improve the surface properties, in this research we prepared polyelectrolyte multilayer (PEM) composed of chitosan and poly-glutamic acid on titanium alloy through spin-coating method, and bone morphogenetic protein 2 (BMP-2) was further loaded. In vitro study using SD rat bone-marrow mesenchymal stem cells was carried out to observe the biological effects. The results showed that the PEM treated surface was well biocompatible. Controlled BMP-2 release reached 5 days. Significant osteogenic differentiation was observed comparing to non-treated group at day14. After 21 day co-culture, we found that the calcium content on PEM group was twice more than non-treat group. The results convinced the facilitated cellular mineralization process. Using polyelectrolyte multilayer to improve the surface property and apply for controlled drug release is of great potential.
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