| Summary: | 碩士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 103 === The objective of this thesis is to construct an ultrasensitive fluorescent platform via bioinspired concept as well as to design and synthesize a short-wavelength fluorescent probes, called AYF. The proposed novel AYF recognize Hg (II) to achieve oxymercuration by the alkyl functional group. Fluorescent molecule(λem=460 nm) and second messenger (two equivalents fluoride ions) are released after a series of reaction, including oxymercuration, hydrolysis andβ-elimination. The conventional LOD of AYF is 1ppm. In order to improve the sensitivity, the thesis mimics the pathway of signal transduction by Organism as well as combines AYF with other probes for detecting fluoride: DCC (λem = 595 nm), DPF1 (λem = 460 nm) and DPF2 to prove hypothesizes of signal transduction and signal amplification. Finally, experimental results demonstrate that the LOD is 0.07 ppb with the proposed combination strategy of AYF, DPF1 and DCC, which is 14000 times lower than the detection method with single probe.
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