The Study on Dual Optical Analytical System for Creatinine and D-lactate

• Main Authors: Ya Li Tsai, 蔡亞歷
• Other Authors: Chien-Ming Chen
• Online Access: http://ndltd.ncl.edu.tw/handle/r8vb74

碩士 === 國立臺北科技大學 === 光電工程系研究所 === 103 === In this thesis, we have developed a novel fluorescent bioassay for creatinine which has advantages of fast detection and low cost. Creatinine will be reacting with 3,5-Dinitrobenzoic acid under the highly alkaline condition. The complexes have fluorescent characteristic that absorb excitation light at 400 nm and release fluorescence at 491 nm. By detecting the fluorescence, we can find out the concertation of creatinine. Thus we used laser diode (LD), spectrometer and 3-D translation stage to construct this fluorescent bio-system. The results show that this fluorescent bio-system have good linearity from 5 μmol/L to 300 μmol/L (R2 = 0.9934) and the limit of detection (LOD) is 5 μmol/L. The accuracy and precision of intra-day ranged from 81.82 to 99.75 % and 7.33 to 10.11 %, respectively. The accuracy and precision of inter-day ranged from 86.75 to 87.85% and 3.08 to 9.39 %, respectively. Also, compared to National Laboratory Animal Center and our bio-system, the mice serum test results show that the correlations (R) are 0.80. In addition, we use the fluorescent bioassay for D-lactate that was previously developed by our labs. To construct a fluorescent bio-system for creatinine and D-lactate assaying. The results show that this fluorescent bio-system have good linearity from 5 μmol/L to 125 μmol/L (R2 = 0.998) and the limit of detection (LOD) is 5 μmol/L. The accuracy and precision of intra-day ranged from 96.22 to 108.01 % and 3.94 to 52.67 %, respectively. The accuracy and precision of inter-day ranged from 95.13 to 101.24 % and 7.65 to 23.86 %, respectively. Also, compared to high performance liquid chromatography (HPLC) and our bio-system, the mice serum test results show that the correlations (R) are 0.69. From this, this fluorescent bio-system provides a rapid, accurate, low cost and convenient for creatinine and D-lactate determination in biological sample.