Chitosan homogenous coating alginate fiber for improving the cell in fiber and promoting the efficiency for drug release

• Main Authors: Tsang-Wen Chang, 張藏文
• Other Authors: 林忻怡
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/vsq3c5

碩士 === 國立臺北科技大學 === 化學工程研究所 === 103 === In tissue engineering, it is important that scaffolds require porosity, biocompatibility and degradability. Alginate is a not only non-toxic but water-solublenatural material to fabricate scaffold. It can uniform mix with cell and provide growth environment, but it is poor for mechanical properties and weakly interact with cell.Chitosan is also good biomaterial. It is made porous 3D-scaffold. Although it have good mechanical properties and better interact with cell, preparations need acid environment. The cell is harmful at acid environment. In order to make better alginate scaffold, we use advantage both biomaterials to make the coating scaffold and we can use it to improve drug release efficiency. In this study, alginate could do ion-crosslinkedwith calcium chloride or chitosan in calcium chloride, and using rapid prototyping systems made of porous scaffold; experimental anti-inflammatory drug diclofenac as the carrying drugs for drug delivery system.Containing drugs and osteoblast scaffold co-cultured with macrophage to study the release properties (drug loading, drug release rate, anti-inflammatory tests, qPCR), and coated with chitosan content , cell viability and toxicity testing are discussed