Research and application of tissue hydrogels from acellular cartilage extracellular matrix

• Main Authors: Ni-Yin Yu, 游妮胤
• Other Authors: 方旭偉
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/r7r8af

碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班 === 103 === Properties of cartilage, an essential connective tissue that is characterized by limited capacity for self-repair, are highly dictated by the composition of extracellular matrix secreted by its chondrocytes. Extracellular matrix (ECM) consists of proteoglycans, polysaccharides, and fibrous proteins that provide a supportive environment for surrounding cells and a physiological framework for cell adhesion, differentiation, and cell-to-cell communication. Because of its significance, many current tissue repair products strive to duplicate the biomechanical properties of ECM in cartilage repair. For instance, hydrogels that it is immobilized defect side instead of periosteum towards for clinical development of scaffold. Tissue adhesive is a type of hydrogel that can be uniformly fill an irregular defect. In this study, we use hydrogels of acellular cartilage extracellular matrix (ACM) as scaffold of adhesive to replace injured cartilage. Hydrogels of ACM are able to avoid immune-mediated rejection while retaining the major natural composition of extracellular matrix, which through a series of acellular program. At an optimum concentration ACMH of 30 mg/mL and 20 mg/mL, our experiments confirm that acellular cartilage extracellular matrix with hydrogel (ACMH) as a scaffold can remove nearly all DNA (99.61%) and retain most of the matrix, such as collagen (78.29%) and glycosaminoglycan (GAG) (61.18%). Turbidimetric gelation kinetic curves for the hydrogels at different ACM concentrations revealed that higher concentrations have shorter gelation time. Rheological characterization of ACMH demonstrates a greater elastic modulus (G’) for a higher concentration. Lastly, as seen in a series of physical tests, ACMH possesses good adhesive quality and adequately fills in cartilage defects. This research has successfully developed a tissue-adhesive hydrogel formulation type of natural acellular cartilage extracellular matrix with hydrogel (ACMH), Hydrogel will be affect by acellular cartilage extracellular matrix concentration, digestive conditions, an acidic environment, pH, ionic strength of salt, temperature and other parameters. It was not easy prepared from organizations, we develop the further discussion and analysis, with natural tissue composition prepared hydrogels want to apply to cartilage tissue engineering with good effect.