Formosan sambar deer velvet antler extract on physical fatigue in murine model

• Main Authors: Jhih-Jie Yu, 余至傑
• Other Authors: Chang-Chi Hsieh
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/31786717469044135542

碩士 === 東海大學 === 畜產與生物科技學系 === 103 === Velvet antler has been used in traditional Chinese medicines and health food over 2100 years. Formosan sambar deer is the indigenous subspecies in Taiwan. Their velvet antler has been steadily used as traditional Chinese medicine and health food more than 400 years in Taiwan. Since 1963, Formosan sambar deer has been started to farming. Today, more than a half of velvet antler source is from Formosan sambar deer in the Taiwan traditional Chinese medicine market. Physical fatigue is a physiological phenomenon that appears with physical stress and exhaustive exercises, usually associated with tissue damage and related energy shortages. This study deals with the improvement effects of Formosan sambar deer velvet antler extract (VA) for the physical fatigue. In this study, male BALB/cByJNarl mice were divided into four groups, the distill water administered control group and the other three groups were administered with VA in 50, 150, 450 mg/kg by gavage every day for two weeks. The results showed that VA supplementation can effectively increase of blood glucose, tissue glycogen, forced swimming time and forelimb grip strength. VA supplementation significantly decreased blood lactic acid, blood urea nitrogen, creatinine phosphate kinase, lactate dehydrogenase, malonaldehyde and 4-hydroxy-trans-2-nonenal. High performance liquid chromatography (HPLC) has confirmed the presence of branch chain amino acid (leucine, isoleucine and valine), carnosine and anserine in VA. Branch chain amino acid, carnosine and anserine as a pH buffer when the decline in blood pH was attenuated, and they can neutralize excess hydrogen ion during high-intensity exercise. VA supplementation increase GLUT-2, CAII, CAIII in liver, and increase GLUT-4, MYOM-3 and TNNI-1 in muscle. VA also decreased TNNI-3 in heart. Taken together, these results suggest that VA can auxiliary antioxidant, glucose transporters, muscle contraction and decreased lactic acid had anti-fatigue activity and could elevate the exercise tolerance.