| Summary: | 碩士 === 東海大學 === 食品科學系 === 103 === Cordyceps militaris is a traditional medicinal mushroom and contains rich bioactive compounds such as cordycepin, adenosine, cordycepic acid, and so on. C. militaris had many pharmacological functions such as anti-inflammatory, antifatigue, antimetastatic, anti-diabetic, anticancer, antiproliferative and anti-leukemia. Light is one of the most important factors impacting growth, metabolism and reproduction of fungal.Different color wavelengths of light can regulate some metabolic pathways to enhance some functional components. LEDs (light-emitting diodes), a good artificial light, have a higher photoelectric conversion efficiency, safety, low heat output, long life, energy saving and without environmental changes. The aim of this study was to investigate the effect of different wavelengths of light of C. militaris fermented brown rice.The biomass reached a maximum of 12 hours (0.49 g / g dw)under fluorescent light. Although adenosine irradiated 8 hours (948.90 ug/g dw) was the highest, but with 12 hours was no difference statistically. In addition, cordycepin and cordycepic acid at most contents for 12 hours, respectively 3974 ug/g and 21.31mg/g dw. Ergothioneine irradiated 16 hours at most (101.52 ug/g dw ). Irradiation of 8 hours could get the highest flavonoid (193.83 ug/g dw).Total phenol content irradiated 4 hours is the best ( 3.63 mg/g dw ). In order to obtain higher biomass and bioactive compounds it would be more suitable to choose irradiation time of 12 hours as a subsequent experiments to explore. Red LED light could be higher biomass (0.38 g / g dw) and cordycepic acid (23.64 mg / g dw) contents. In terms of blue LED light could get a higher adenosine (760.10 ug/g dw) and flavonoids (216.31 ug/g dw) of contents. Green LED irradiated cordycepin (48.97 ug/g dw) and ergothioneine (2894.70ug/g dw) could be obtained higher levels. Total phenol content under the fluorescent light of the contents may be high (1.83 mg/g dw). Results above the showed the different wavelength of LEDs which were better than traditional fluorescent. LEDs effect biomass, bioactive compounds and antioxidants ability of C. militaris fermented brown rice. Therefore, subsequent experiments to select the preferred conditions of the two LED fixed wavelengths of the light in different proportions to explore, such as the LED (red: blue) combination and LED (blue: green) combination as sources. Mixed (red: blue) of (R5B1) LED light wavelengths of the biomass may be higher (0.56 g /g). And mixed in different proportions (red: blue) of (R2B4) LED light wavelengths could obtain high levels of adenosine (5451.79 ug/g dw mycelia), cordycepic acid (86.52 mg / g dw mycelia) and total phenolic content (8.23 mg/g dw mycelia). In terms of different proportions (red: blue) of (R3B3) LED light wavelengths could be higher of cordycepin (30.03 mg / g dw mycelia) and ergothioneine contents (387.03 ug/g dw mycelia). Blue LED and mixed (blue: green) of (B2G4) LED light wavelengths could have a high flavonoid content (637.47 ug/g dw mycelia). Based on the above results showed that mix (red: blue) combined in a ratio of the LED light source caused the most effect on biomass, bioactive compounds and antioxidants of C. militaris fermented brown rice. Green LED influenced less. In conclusion, the different proportions LED light wavelengths could regulate biomass, bioactive compounds and antioxidants of C. militaris fermented brown rice. Future of adjusting of light wavelengths can be used to increase the C. militaris fermented brown rice of functional contents and related development of health food.
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