| Summary: | 碩士 === 東海大學 === 生命科學系 === 103 === Cell migration is an important process which is involved in many biological functions. Focal adhesion, an integrin containing protein complex connected with extracellular matrix and cytoskeleton, plays the major role during cell migration. It has been reported that integrin is transported to cell membrane through vesicular trafficking and been regulated by Rab11, the endosome traffic protein. But how Rab11 been regulated is still unknown. Mob2, which has been first found in Saccharomyces cerevisiae, conserved from yeast, drosophila and mammals to human and is reported to regulate vesicular trafficking through Rab GTPase. Therefore, in this study, we investigated whether Mob2 could regulate sarcoma cell migration through Rab11 mediated integrin recycling. Results showed that Mob2 knockdown inhibited fibrosarcoma cell (HT1080) migration, and Mob2 regulated integrin recycling during the cell polarization. We further demonstrated Rab11 knockdown in Mob2 overexpressed cells inhibited cell migration; however, Rab11 overexpression in Mob2 knockdown cells has no effect on cell migration. This study also showed that miR758 overexpression induced Rab11 and Mob2 expression. Taken together, Mob2 may regulate Rab11-mediated integrin recycling for fibrosarcoma cell migration, and miR758 might play as a upstream regulator of Mob2, however, their causal relationship needs to be further investigated.
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