Protective Effect of Q10 on Rat Hearts of Doxorubicin Induced CardiomyopathyEffect of K.pneumoniae Infection on Hearts of Diabetic BLAB/c Mice

• Main Authors: Zhao-Rong Liu, 劉炤榮
• Other Authors: Chih-Yang Huang
• Format: Others
• Language: en_US
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/5j7umt

碩士 === 亞洲大學 === 生物科技學系 === 103 === Doxorubicin is a widely chemotherapeutic drug for treat-ing cancer, but doxorubicin is dose dependent to damage heart cause cardiac toxicity. Q10 might effectively reduce cardiotoxicity caused by doxorubicin, to protect the function of the heart, often used in the medical nutritional supplements on cancer treatment. This study focuses on previously used Q10 whether can achieve the prevention of cardiovascular disease caused by doxorubicin treatment. 4 weeks SD rats adapt lab for 4 weeks as animal model and randomly divided into control, doxorubicin, oral Q10 treatment by doxorubicin (Dox+Q10) and Q10 four groups, control group is oral water 10 ml/kg every day and IP physio-logical saline, doxorubicin groups is IP doxorubicin 2.5 mg/kg every three days and Q10 groups is oral Q10 10 mg/kg every day, all groups treat 3 weeks and then animal sacrifice, analysis heart weight by one way ANOVA statistics and western blot analysis TGF β1, CTGF, COL1A1, MMP-9 and MMP-2 in fibrosis pathway, Bak, Bax, cytochrome c, caspase-9 and caspase-3 in apoptosis and p-PI3K, p-Akt, p-Bad, Bcl-2 and Bcl-xL in survival by mitochondria dependent pathway, Fas-L, FADD and caspase-8 in cell death pathway. Found heart of doxorubicin damage caused decreased heart weight, cardiomyocytes arrangement disarray and numerous of collagen accumulation, enhanced fibrosis pathway and cell death pathway, inhibited survival protein to cause cardiomyocytes apoptosis. However, advance use Q10 then damaged by doxorubicin, Q10 can protect cardiomyocytes and reduce fibrosis pathway and cell death pathway protein expression, enhance sruvival protein expression and inhibit cardiomyocytes apoptosis. Therefore, Q10 can indeed found to prevent heart damage caused by doxorubicin achieve a protective effect. Diabetes is a metabolic disorder and associated with a variety of organs and cardiovascular diseases. Klebsiella pneumoniae is a Gram-negative bacterium, low immunity sus-ceptible to Klebsiella pneumoniae infection and the formation of sepsis. Hospitalized diabetic patients because they would have immunity problems and susceptible strains of nosocomial in-fections, Klebsiella pneumoniae as the main bacteria in noso-comial infections to cause species, infected patients lead to a variety of organ failure and septic shock caused by sepsis, and diabetes patients often complicated by cardiovascular disease, sepsis caused by infection can destroy the mitochondria of cardiac cells to produce cell apoptosis, resulting in cardiac dysfunction, this study aims to explore diabetes infection by Klebsiella pneumoniae weather in addition of cardiac damage. 8 weeks BLAB/c male mice first divided normal and DM groups, DM group IP STZ 55 mg/kg to induce type I diabetes and normal group IP saline for 5 days, then when 15-16 weeks will randomized each groups selected one group to oral infec-tion 105 CFU of Klebsiella pneumoniae for 3 days and then animal sacrifice, analysis heart weight by one way ANOVA statistics and western blot analysis p-Akt and Bcl-2 survival proteins. Found serious narrowing in heart weight and addition of inhibit survival protein expression in diabetes infection by Klebsiella pneumoniae, observed cardiomyocytes arrangement disorder by H&E stain and numerous collagen accumulation in heart tissue by Masson’s trichrome stain, left ventricular sys-tolic diameter, ventricular wall thickness, systolic rate and blood injection rate all decrease by echocardiography, proof Klebsiella pneumoniae infection in diabetic mice have addition of heart damage, even cause heart function of an obstacle. Therefore, diabetic patients prevent nosocomial infection in hospital medical care is a very important issue.