| Summary: | 碩士 === 亞洲大學 === 生物科技學系 === 103 === Dictyophora indusiata belonging to Basidiomycota, Agaromycetes, Phallales, Phallaceae and Dictyophora , is an edible mushroom widely used in Asian countries such as Indonesia, Malaysia, India, Southern Japan, China and Taiwan. It was called “veiled lady mushroom” or “queen of the mushrooms” due to its beautiful appearance, delicious taste and health function. Many studies suggested that D. indusiata polysaccharide is β-d-glucan. This natural polysaccharide possessed excellent potential bioactivities such as anticancer, antitumor, immunomodulation activity, antioxidant activities, and so forth. Although its edible and medicinal value was extremely high, however the yield produced in Taiwan is rarely, mostly rely on importing from China. D. indusiata imported from China was expensive and added an excess of sulfur dioxide. This study was to investigate the D. indusiata mycelial growth and fruiting body development on agar plate medium and sawdust substrate, preserved fresh fruiting bodies of D. indusiata and tried to find out the most optimum methods in cultivation. Results showed the best environment for mycelial growth on PDA medium was 26℃ and pH 5.5. On sawdust substrate, results showed the best temperature for mycelial growth were 28℃. The suitable substrate formulations were to add 20% wheat middling, with small amount of CaSO4 and sucrose. Studies on sawdust medium substituted with Moso bamboo shavings or paddy straw. Showed the mycelium of D. indusiata grew faster by Moso bamboo shavings but slower by paddy straw. In this study, substrate formulation of sawdust(78%), wheat middlings(20%), CaSO4(1%) and sucrose(1%) have the higher mycelial growth. Alternative suitable formulations were sawdust(19.5%), bamboo shavings(58.5%), wheat middlings(20%), CaSO4(1%) and sucrose(1%) showed the highest mycelial growth. Test on fruiting development revealed that mycelium grown in substrates which covered with sawdust and organic fertilizer produced the highest yield. The gulei stored at 4℃ still could develop the normal fruiting body at room temperature. The fruiting body of D. indusiata treated with 1000 ppm Vitamins C solution for 1 minute prolonged its shelf life 10 days.
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