| Summary: | 碩士 === 靜宜大學 === 食品營養學系 === 104 === Hyperglycemia-induced oxidative stress may lead to kidney injury, and eventually end-stage renal disease (ESRD). The anti-oxidant activities of fermented red bean extract (FRBE) have been reported in in vitro studies by measuring the DPPT assay, Fe (2+)-chelating ability, and reducing power. However, its effects on diabetic nephropathy are still unknown. In this study, we examined the anti-oxidative and anti-inflammatory effects of FRBE in kidneys of STZ-induced diabetic rats. Forty Wistar rats were randomly assigned into five experimental groups, that is healthy control, diabetic control, diabetic rats with low dose FRBE (100mg/kg BW), diabetic rats with medium dose FRBE (200mg/kg BW) and diabetic rats with high dose FRBE (500mg/kg BW). Normal control rats and diabetic rats were gavaged with saline and FRBE respectively for 12 weeks and then sacrificed. Concentration of fasting glucose, adiponectin, renal function (BUN, Scr, Ucr, Ccr and UAE), anti-oxidative markers (ROS、AGE、GSH、GPx and SOD) and pro-inflammatory markers (NF-kB, IL-6,TNF-α, MCP-1 and CRP ) were measured. The results of this study showed that FRBE can effectively inhibit the formation of renal ROS and AGEs, and increase renal GSH content and serum adiponectin. Fasting glucose and renal IL-6 concentrations was decreased by low dose FRBE. Serum CRP, renal TNF-α and MCP-1 concentrations were decreased and renal SOD activity was increased in medium dose FRBE group. In conclusion, these results suggest that 200mg/kg BW of FRBE could attenuate diabetes associated oxidative and inflammatory stress in kidneys of diabetic rats.
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