Neuroprotective Effects of Glechoma Hederacea Extracts against Oxidative Damage and Its Underlying Mechanism in PC12 Cells

• Main Authors: Wan-Ching Chan, 詹菀菁
• Other Authors: Su-Tze Chou
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/92004778626310796935

碩士 === 靜宜大學 === 食品營養學系 === 103 === A number of studies have indicated that reactive oxygen species (ROS) are involved in the age-related chronic degeneration in Alzheimer’s disease (AD) characterized by a progressive loss of cognitive function. Recently, there is an increasing interest in the study how to prevent or delay the AD development. Glechoma hederacea belongs to Lamiaceae family, is used for medication purpose widely. In this study, we examined the neuroprotective effects of hot water extract of Glechoma hederacea (HWG) and its possible mechanisms in H2O2-treated rat adrenal medulla pheochromocytoma cells (PC12 cells). The results demonstrated that the HWG had acetylcholinesterase (AChE) inhibitiory activity and was a competitive cholinesterase inhibitor. In the cell model, the HWG didn’t possess the cytotoxicity in PC12 cells and can recover the cell morphology and the cell viability of H2O2-treated PC12 cells. HWG can rescue the cells from H2O2-induced apoptosis and DNA damage which was confirmed by DAPI stain and comet assay, respectively. Flow cytometry assay revealed that HWG can decrease the apoptotic cell population, ROS production and caspase-3 activity and increase the mitochondrial membrane potential (MMP). Also, our results indicated that the distribution of cytochrome c and AIF were attenuated which were confirmed by FITC immunofluorescent stain in H2O2-treated PC12 cells after HWG treatment. In addition, HWG can improve the glutathione (GSH) levels, decrease the malondialdehyde (MDA) levels and regulate the glutathione peroxidase (GSH-Px)、superoxide dismutase (SOD) and catalase (CAT) activities to the normal levels in H2O2-treated PC12 cells. In conclusion, HWG possessed the AChE inhibitiory activity and increased the growth in H2O2-treated PC12 cells. HWG inhibited the cell apoptosis and DNA damage, decreased the apoptotic cell population, ROS production and caspase-3 activity, increased the MMP and balanced the oxidant-antioxidant defense systems in H2O2-treated PC12 cells. Our results suggest that HWG may have the antioxidant activity and neuroprotective potential.