| Summary: | 碩士 === 中國文化大學 === 化學工程與材料工程學系奈米材料碩士班 === 103 === Bio-ethanol can prepare by decomposing starch or cellulose into glucose units ingredients then fermented to produce bio-ethanol . The experiment is miscanthus as ethanol material, due to the nature of matter Wo miscanthus as lignin and cellulose with a lot of living to do the quality of the base alcohol , and a lot of other fast-growing , as the material for ethanol production is excellent choise.
Kraft pretreatment cooking method is used in this experiment . First , NaOH and Na2S 25% sulfidity , 20% active alkalinity to configure the cooking liquid , then take miscanthus powder and pieces to liquid three different ratio of adding cooking liquid cooked to 150-165 ℃ for 3 hours, cellulose and lignin cooking Miscanthus decomposition, destruction of the polymer structure of cellulose and lignin formation of small units link .
Added fiber and fiber hydrolysing enzyme saccharification enzymes , placed in a constant temperature water bath to 40 °C for 72 hours saccharification reaction of glucose every 24 hours to measure its sugar content and record the change , and finally the yeast was added an aqueous solution of the fermentation reaction , turn into bio-ethanol . Brix measurements of changes in saccharification reaction , and measuring the ratio of the aqueous solution of glucose sugar content . Finally, measuring the biomass produced by the fermentation of alcohol and the resultant ratio.
KEY WORDS: Miscanthus , Kraft Pretreatment , bio-ethanol, enzymatic hydrolysis , fermentation
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