| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 103 === Diamond is an allotrope of carbon. A unique property that distinguishes it from the other carbon materials is that diamond is optically transparent and often contains point defects as color centers. Nitrogen vacancy (N-V) defects are the most noteworthy color centers in diamond, because it emits far-red fluorescence with high photostability. This unique optical property combined with the non-cytotoxicity and good surface functionalizability characteristics of the material makes nanoscale diamonds a promising fluorescent probe for biolabeling applications in cellular environments.
Finally, We have successfully determined the quantity of cellular uptake of bare FNDs and BSA-conjugated FNDs in HeLa cells with both flow cytometry and fluorescence spectroscopy. As a proof of the results, we have conducted the confocal fluorescene miscroscopy for HeLa cells incubated with bare FNDs and/or BSA-coated FNDs. Clearly, there are still many things to explore with surface-modified FNDs. It is anticipated that the surface functionalized fluorescent nanodiamond will meet up with several biological and medical needs in the next few decades to come.
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