Preventive Effect of Bracteanolide A and Longan Flower Extracts on Hepatic Protection of Different Rat Liver Injury Models

• Main Authors: Cheng-Chu Hsieh, 謝政橘
• Other Authors: 吳應寧
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/17944906748125816697

博士 === 國立臺灣大學 === 獸醫學研究所 === 103 === Liver injury, which can be caused by liver surgery, liver transplantation, and diseases such as metabolic syndrome (MetS), is considered an important medical issue in Taiwan. The increasing prevalence of MetS in recent years has been tightly associated with the development of abnormal liver metabolism and hepatocellular carcinoma. Thus, our current studies focus on the protection of hepatic injury. In this study, we used proteomic approaches to investigate the preventive effects of longan flower water extract (LFWE) on high fructose (HF)-induced MetS in rats. The results show that fructose metabolism, glycolysis, lipogenesis, endoplasmic reticulum stress, inflammatory activities, fatty acid oxidation and antioxidant capacity are significantly altered in rats with MetS; however, the abnormal protein expression pattern could been rescued with LFWE supplementation. We further investigated the protective effects of longan flower ethyl acetate extract (LFEE) and Bracteanolide A pretreatment in a rat hepatic I/R injury model. The results display that both LFEE and Bracteanolide A can ameliorate I/R injury-mediated increase of aspartate aminotransferase, alanine aminotransferase, interleukin-1β, interleukin-6, interleukin-10, tumor necrosis factor-α, inducible nitric oxide synthase, and cyclooxygenase-2 activities, while at the time elevating hepatic glutathione levels after I/R injury. Histological results indicate that both LFEE and Bracteanolide A are effective in improving liver morphology during I/R injury. Therefore, LFEE and Bracteanolide A might offer a novel intervention for patients suffering from hepatic I/R injury caused by hepatectomy and liver transplantation.