| Summary: | 碩士 === 臺灣大學 === 獸醫學研究所 === 103 === Articular cartilage does not contain blood vessels, so it does not have the ability to regenerate and repair. Therefore, in tissue engineering studies, materials that promote articular cartilage regeneration are very important. Sodium hyaluronate is one of the critical molecules for the maintenance of the physico-chemical characteristics of cartilage extracellular matrix. Mesenchymal stem cells are multipotent stem cells and can differentiate into a variety of cell types, involving: adipocytes, chondrocytes, and osteoblasts. We want to know the effect of intra-articular injection of hyaluronan plus bone marrow stem cells (BMSC) promote cartilage regeneration in the treatment of osteoarthritis.
In this study, an injectable sodium hyaluronate combine bone marrow stem cells (BMSC) was used for articular cartilage regeneration. Female 16-week-old New Zealand white rabbits were divided into four groups for treatment (n=6 per group): (1) control group - no treatment; (2) injected BMSC 3×106 cells/0.5 ml PBS; (3) injected sodium hyaluronate 0.1ml/kg; (4) injected sodium hyaluronate 0.1ml/kg combine BMSC 3×106 cells/0.2 ml PBS.
Each animal underwent surgery to create full thickness cartilage defects on the articular surface of the medial femoral condyle of right stifle joint. The defects were 3 mm diameter and 2 mm depth. At 1 and 2 weeks after surgical, experiment material was injected into the right articular capsule of each rabbit in the transplanted group. At 14 weeks after surgical, the regenerated cartilage was examined using the International Cartilage Repair Society (ICRS) Macroscopic Score and the ICRS Visual Histological Score.
A statistically significant difference between the experimental and control groups was observed. The forth group closest to the normal cartilage tissue structure, then followed by the third group. It is a promising therapeutic approach to joint damage. The combination of BMSC and HA yield good results and are beginning to gain recognition in regenerative medicine.
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