The Effect of Patelet-rich Fibrin (PRF) Releasate Combines with Bone marrow-derived Mesenchymal Stem Cells on Type I Diabetes Mellitus with a Mouse Model

• Main Authors: JunJie Huang, 黃俊杰
• Other Authors: Tzong-Fu Kuo
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/35801456600852919957

碩士 === 臺灣大學 === 獸醫學研究所 === 103 === Diabetes Mellitus is a serious disease in which the body cannot properly control the amount of sugar in your blood because it does not have enough insulin. And it can be divided into Type I diabetes and Type II diabetes. Type I diabetes mellitus is a chronic disease characterized by the autoimmune destruction of insulin-secreting pancreatic beta-cells, which results from the body''s failure to produce enough insulin and gives rise to fasting and postprandial hyperglycemia. The classic symptoms are polyuria (excessive urination), polydipsia (excessive thirst), and polyphagia (excessive hunger). Stem Cells are an unspecialized cell that gives rise to differentiated cells. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells which can differentiate into cardiac cells, chondrocytes, adipocytes and so on. And mesenchymal stem cells can differentiate into differentiate into specific cells under different conditions. Platelet-rich fibrin (PRF) is a source of autogenic neotype biomaterial, which is rich in cytokine and growth factors, and is called new generation of platelet concentrates. The molecular constitution of PRF is similar to nature blood clot, and to provide location with migration, proliferation, and differentiation for rhagiocrine cell. Currently, the slow polymerization during PRF preparation seems to generate a fibrin network very similar to the natural one. Such a network leads to a more efficient cell migration and proliferation and thus cicatrization. In our research, the mesenchymal stem cells derived from mouse bone marrow and adipose can be induced to proliferate and differentiate into islet-like cell clusters and to form islet-like structure. The stem cell differentiated islet could ameliorate the glyco metabolic disorder in diabetic animals and the result is as good as the group which use mesenchymal stem cells with PRF releasate.